Audiovisual Exercise On Cdna Cloning And Characterization

SWANSEA UNIVERSITY - College of Medicine PM-305: Gene Manipulation [DHJ] Assignment: Audiovisual Exercise on cDNA Cloning and Characterisation. This is not a test, but it is a learning exercise designed to make you think about the use of some gene manipulation techniques. During the course of this video demonstration you will be asked the following questions. Each number indicates a different place when the programme will be stopped. Each question is related to the material covered before that stop. You will be given time to think about each answer and make a brief note of it. Any questions which are not directly answered when the programme is re-started will be addressed at the end of the session. You must then make a full record of questions and answers for your continuous assessment. (Question 12 is to be done afterwards. Note the need for diagrams and see submission instructions at the bottom of Page 2.) QUESTIONS: 1. Only cDNA cloning is covered in this video. What other methods are there for obtaining a cloned coding sequence? The method we saw in the video was cloning of a cDNA library. A cDNA library involves the physical cloning of the thaumatin gene by the cDNA fragment being inserted into a suitable vector called a plasmid. This method of cDNA cloning means that only the desired gene is cloned and colonies contain the plasmid can be identified with techniques like the blue white screening. There are other methods we can use though. For instance we could make a